Sex identification of neotropical macaws (Ara spp.) from invasive and non-invasive samples: Identificación del sexo en guacamayas neotropicales (Ara spp.) a partir de muestras invasivas y no invasivas

Lady Johana  Franco-Gutiérrez; Jóhnatan  Álvarez-Cardona; Iván Darío  Soto-Calderón

doi:10.59517/oc.e389

Authors

Lady Johana Franco-Gutiérrez Laboratorio de Genética Molecular (GENMOL), Universidad de Antioquia
Jóhnatan Álvarez-Cardona Parque Zoológico Santa Fe
Iván Darío Soto-Calderón Laboratorio de Genética Molecular (GENMOL), Universidad de Antioquia

DOI:

https://doi.org/10.59517/oc.e389

Keywords:

CHD, cromosomas sexuales, muestreo no invasivo, identificación del sexo, Psittacidae

Abstract

Aunque la amplificación del gen Helicasa con Cromodominio de Unión a ADN (CHD) ha sido usado para inferir el sexo en guacamayas a partir de sangre y plumas, el uso de muestras no invasivas ha sido poco explorado. Aquí optimizamos un protocolo completo de identificación molecular del sexo en guacamayas (Ara spp.) a partir de muestras de sangre, plumas arrancadas y muestras fecales, utilizando los primers 2550F/2718R para amplificar el gen CHD. Este protocolo es versátil y de utilidad para estudios tanto ex situ como in situ. Logramos identificar exitosamente el sexo de las guacamayas a partir de los tres tipos de tejidos muestreados. Sin embargo, recomendamos el uso de deposiciones fecales frescas para minimizar la manipulación, el estrés y el riesgo de lesión de los animales.

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